Potential elicitors on secondary metabolite production and antioxidant defence activity of two tomato (Solanum lycopersicum L.) varieties

Submitted: 28 March 2021
Accepted: 7 June 2021
Published: 6 July 2021
Abstract Views: 1693
HTML: 57
PDF: 533
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Plants protect themselves, after pathogen attack, through the passive and active defence mechanisms. The treatment of plants with various agents, including cell wall fragments, plant extracts, and synthetic chemicals, can induce resistance to subsequent pathogen attack both locally and systemically. In view of the capability of phytochemicals compounds found in fruits and vegetables with different proven health benefits to consumers, there are different strategies to enhance the concentration of these compounds, among which the use of elicitors. Elicitation has been used to trigger different defence responses in plants, which lead to different mechanisms, such as activation of enzymes related with secondary metabolism. In this study we investigated the effects of elicitors (Activane®, Micobiol®, Stemicol® in doses of 1.8 g L–1, 3 m L–1 and 2.5 g·L–1) on the activity of two enzymes (polyphenoloxydase and peroxidase) in leaves as well as on the bioactive content (DPPH scavenging activity, total phenol and total flavonoid content) in fruits of Solanum lycopersicum L. The elicitors studied affected the production of enzyme and antioxidant activities in tomato, though showed a variable influence on the production of total phenolic and flavonoid content in tomato fruits. In general, the elicitors are able to increase polyphenoloxydase activity in leaves and phytochemical properties in fruits, with a significant negative correlation between the DPPH scavenging activity, total phenolic and flavonoids. These findings provide positive directions for the possible use of these elicitors in tomato (‘Bola’ and ‘Saladette’) production in greenhouse.

Highlights
- Elicitors increase the enzymatic activity of PPO in tomato leaves.
- The production of total phenolic contents and flavonoids depends on type of elicitors.
- No effects on the peroxidase content of the tomato leaves.

- The antioxidant capacity of the aqueous fruits extract showed a significant difference (P=0.05) between the treatments in the Saladette variety.
- Positive relationship between total phenolic contents and flavonoids.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Ãvila-Juárez L, Miranda-Rodríguez H, 2018. Variations in bioactive content in different tomato trusses due to elicitor effects. J. Chem. (Hindawi), 1-9. DOI: https://doi.org/10.1155/2018/2736070
Baenas N, García C, Moreno DA, 2014. Elicitation: a tool for enriching the bioactive composition of foods. Mol. 19:13541-63. DOI: https://doi.org/10.3390/molecules190913541
Chakraborty N, Ghosh S, Chandra S, Sengupta S, Acharya K, 2016. Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison. Physiol. Mol. Biol. Plants. 22:307-20. DOI: https://doi.org/10.1007/s12298-016-0373-z
De la Rosa LA, Floresa AV, Parrilla E, Garcıa J, Campos ON, Nava A, Reyes S, Chaverri JP, 2014. Content of major classes of polyphenolic compounds, antioxidant, antiproliferative, and cell protective activity of pecan crude extracts and their fractions. J. Funct. Foods. 7:219-28. DOI: https://doi.org/10.1016/j.jff.2014.02.008
FIRA (Trusts Established in Relation to Agriculture), 2019. Red tomato agri-food panorama. Available from: diees@fira.gob.mx
Fritig B, Legrand M, 1993. Mechanisms of plant defence responses. In Fritig B, Legrand M (Eds.), Developments in plant pathology. Springer, Dordrecht, 2:0929-1318 DOI: https://doi.org/10.1007/978-94-011-1737-1
Gadzovska S, Maury S, Delaunay A, Spasenoski M, Hagège D, Courtois D, Joseph C, 2013. The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L. Plant Cell Tissue Organ Cult. 113:25-39. DOI: https://doi.org/10.1007/s11240-012-0248-0
Gadzovska S, Tusevski O, Maury S, Delaunay A, Joseph C, Hagège D, 2014. Effects of polysaccharide elicitors on secondary metabolite production and antioxidant response in Hypericum perforatum L. shoot cultures. Sci. World J. 10:609-49. DOI: https://doi.org/10.1155/2014/609649
García E, Robledo A, Mendoza B, Solís G, González SM, 2018. Efecto de elicitores de origen natural sobre plantas de tomate sometidas a estrés biótico. Rev. Mex. Cienc. Agríc. 9:20.
Garcia-Brugger A, Lamotte O, Vandelle E, Bourque S, Lecourieux D, Poinssot B, Wendehenne D, Pugin A, 2006. Early Signalling Events Induced by Elicitors of Plant Defenses. MPMI. 19:7. DOI: https://doi.org/10.1094/MPMI-19-0711
Gorni PH, Pacheco AC, Lima MA, 2020. Elicitation improves the leaf area, enzymatic activities, antioxidant activity and content of secondary metabolites in Achillea millefolium L. grown in the field. J. Plant Growth Regul. 09:23. DOI: https://doi.org/10.1007/s00344-020-10217-x
Goya AK, Middha SK, Sen A, 2010. Evaluation of the DPPH radical scavenging activity, total phenols and antioxidant activities in Indian wild Bambusa vulgaris ‘Vittata’ methanolic leaf extract. J. Nat. Pharm.1:2229-5119. DOI: https://doi.org/10.4103/2229-5119.73586
Hahn MG, 1996. Microbial elicitors and their receptors in plants. Annu. Rev. Phytopathol. 34:387-412. DOI: https://doi.org/10.1146/annurev.phyto.34.1.387
Hawrylak-Nowak B, Dresler S, Rubinowska K, Matraszek-Gawron R, 2021. Eliciting effect of foliar application of chitosan lactate on the phytochemical properties of Ocimum basilicum L. and Melissa officinalis L. Food Chem. 342:128-358. DOI: https://doi.org/10.1016/j.foodchem.2020.128358
Kainama H, Fatmawati S, Santoso M, Ersam T, 2020. The relationship of free radical scavenging and total phenolic and flavonoid contents of Garcinia lasoar PAM. Pharm. Chem. J. 53:1151-7. DOI: https://doi.org/10.1007/s11094-020-02139-5
Mandal S, Kar I, Mukherjee KA, Acharya P, 2013. Elicitor-induced defence responses in Solanum lycopersicum against Ralstonia solanacearum. Sci. World J. 561056:1-9. DOI: https://doi.org/10.1155/2013/561056
Mohamed HI, Mohammed AH, Mohamed NM, Ashry NA, Zaky LM, Mogazy AM, 2021. Comparative effectiveness of potential elicitors of soybean plant resistance against spodoptera littoralis and their effects on secondary metabolites and antioxidant defense system. Gesunde Pflanzen, pp. 1-13. DOI: https://doi.org/10.1007/s10343-021-00546-6
Moreno-Escamilla JO, Alvarez-Parrilla E, Rosa LA, Núñez-Gastélum JA, González-Aguilar GA, & Rodrigo-García J, 2017. Effect of different elicitors and preharvest day application on the content of phytochemicals and antioxidant activity of butterhead lettuce (Lactuca sativa var. capitata) produced under hydroponic conditions. J. Agric. Food Chem. 65:26. DOI: https://doi.org/10.1021/acs.jafc.7b01702
Pandey VP, Awasthi M, Singh S, Tiwari S, Dwivedi UN, 2017. A comprehensive review on function and application of plant peroxidases. Biochem. Anal. Biochem. 6:308. DOI: https://doi.org/10.4172/2161-1009.1000308
Perea-Domínguez XP, Hernández-Gastelum LZ, Olivas-Olguin HR, Espinosa-Alonso LG, Valdez-Morales M, Medina-Godoy S, 2018. Phenolic composition of tomato varieties and an industrial tomato by-product: free, conjugated and bound phenolics and antioxidant activity. J. Food Sci. Technol. 55:9. DOI: https://doi.org/10.1007/s13197-018-3269-9
Pérez-Balibrea S, Moreno DA, García-Viguera C, 2011. Improving the phytochemical composition of broccoli sprouts by elicitation. Food Chem. 129:35-44. DOI: https://doi.org/10.1016/j.foodchem.2011.03.049
Peschel W, Sánchez-Rabaneda F, Diekmann W, 2006. An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chem. 97:1. DOI: https://doi.org/10.1016/j.foodchem.2005.03.033
Reimers PJ, Guo A, Leach JE, 1992. Increased activity of a cationic peroxidase associated with an incompatible interaction between Xanthomonas oryzae pv oryzae and rice (Oryza sativa). Plant Physiol. 99:1044-50. DOI: https://doi.org/10.1104/pp.99.3.1044
Rohwer CL, Erwin JE, 2008. Horticultural applications of jasmonates: a review. J. Hortic. Sci. Biotechnol. 83:283-304. DOI: https://doi.org/10.1080/14620316.2008.11512381
Ruiz-García Y, Gómez-Plaza E, 2013. Elicitors: a tool for improving fruit phenolic content. Agriculture. 3:1. DOI: https://doi.org/10.3390/agriculture3010033
Ruiz-García Y, Romero-Cascales I, Gil-Muñoz R, Fernández-Fernández JI, López-Roca JM, Gómez-Plaza E, 2012. Improving grape phenolic content and wine chromatic characteristics through the use of two different elicitors: Methyl jasmonate versus benzothiadiazole. J. Agric. Food Chem. 60:1283-90. DOI: https://doi.org/10.1021/jf204028d
Salas-Pérez L, Gaucín-Delgado J, Preciado-Range P, Gonzales-Fuentes J, Ayala-Garay A, Segura-Castruita M, 2018. La aplicación de ácido cítrico incrementa la calidad y capacidad antioxidante de germinados de lenteja. Rev. Mex. Cienc. Agríc. 20:1. DOI: https://doi.org/10.29312/remexca.v0i20.999
Sbaihat L, Takeyama K, Koga T, Takemoto D, Kawakita K, 2015. Induced resistance in solanum lycopersicum by algal elicitor extracted from Sargassum fusiforme. Sci. World J. 870520:9 pp. DOI: https://doi.org/10.1155/2015/870520
SIAP (Agri-Food and Fisheries Information Service), 2019. Available from: https://www.gob.mx/siap/acciones-y-programas/produccionagricola-33119
Thakur M, Sohal BS, 2013. Role of elicitors in inducing resistance in plants against pathogen infection. Biochemistry 762412. DOI: https://doi.org/10.1155/2013/762412
Tumpa FH, Khokon MAR, 2020. Foliar application of chitosan and yeast elicitor facilitate reducing incidence and severity of Alternaria leaf blight of tomato and brinjal. Res J Plant Pathol. 3:2-4.
Vasconsuelo A, Boland R, 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci. 172:861-75. DOI: https://doi.org/10.1016/j.plantsci.2007.01.006

How to Cite

Cham, A. K., Zacarías, M. del C. O. ., Saldaña, H. L. ., Vázquez Alvarado, R. E. ., Olivares Sáenz, E. ., Martínez-Ávila, G. C. ., & Alvarado Gómez, O. G. . (2021). Potential elicitors on secondary metabolite production and antioxidant defence activity of two tomato (<em>Solanum lycopersicum</em> L.) varieties. Italian Journal of Agronomy, 16(3). https://doi.org/10.4081/ija.2021.1883